⚗️ Assay DF Ratio
Quick Concept & Beginner Tips
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What is the Assay DF Ratio?
When a sample and standard are diluted by different amounts before injection, their peak areas can't be compared directly. The DF Ratio corrects for this difference so the final Assay % reflects true relative potency, not just a difference in dilution.
DF Ratio = Sample Total DF ÷ Standard Total DF
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Why is Weight Correction Needed?
If the exact weight of Standard and Sample weighed out differ, the raw peak area ratio alone will be skewed. Entering both weights applies a correction so the result reflects true potency rather than a weighing difference.
Weight Ratio = Standard Wt ÷ Sample Wt
💡 Beginner Tip — Weights & Purity are optional
With weights entered: Full formula is used — Area Ratio × Weight Ratio × DF Ratio × Purity ÷ 100 × 100.
Without weights: A simplified calculation is used — Area Ratio × DF Ratio × 100. Fill in Standard/Sample Weight and Purity whenever they're available for the most accurate result.