🧮 Dilution Factor Calculator
Quick Concept & Beginner Tips
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What is a Dilution Factor?
Dilution Factor (DF) tells you how many times a solution has been diluted. It is the number by which a sample or standard has been diluted during preparation before analysis.
DF = Final Volume ÷ Aliquot Volume Taken
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What is an Aliquot Volume?
An aliquot volume is the measured portion of a solution that you take from one container and transfer to another for further dilution or testing. Aliquot = The volume you take out.
Example: Take 5 mL → Make to 50 mL → DF = 10
💡 Beginner Tip — What counts as a dilution step?
Include: Volumetric flask make-up, aliquot transfers, further dilutions before injection.
Do NOT count: Weighing, sonication, filtration alone, centrifugation, HPLC injection volume.